Testudo Kleinmanni/werneri

[tortadise]

I have seen a lot of these species(SSP) thrown around lately. So I wanted to start this topic on the discussion. It will be pretty short and I will follow with a phylogenic findings and cited work that was done on various specimens to compare and substantiate if at all possible the Israeli (known as werneri,AKA Negev tortoise) is in fact a different species, or even a sub-species. Unfortunately it failed showing not enough haplotype differences to elevate this "species" to its own or sub species category. Yes it is a known locale species of Kleinmanni, but then again the natural range of Kleinmanni species is very sparse and blotchy along the coast of Mediterranean anyways.

As noted the scientific findings back in 2001;

"Sequence variation of a 1066 bp long mtDNA fragment (cytochrome b gene, adjacent part of tRNA-Thr gene) of four known-locality samples of Testudo kleinmanni (Tripolitania, Libya) and of four samples of T. werneri (Negev, Israel) is compared with additional five sequences of pet trade tortoises allegedly representing T. kleinmanni. Four haplotypes, differing in one to four mutation steps occur. The most common haplotype was shared by all known-locality samples of T. kleinmanni and three T. werneri. Sequence variation within each nominal species and in the pooled sample of T. kleinmanni, T. werneriand pet trade tortoises is the lowest known for any Testudo species. We conclude there is no support for the validity of T. werneri Perälä, 2001."

Bibliography/citing

1. Baha el Din S. 2006. A Guide to the Reptiles and Amphibians of Egypt. The American University in Cairo Press, Cairo and New York, xvi, 359 pp.
2. Bour R. 1996. Une nouvelle espèce de tortue terrestre dans le Péloponnèse (Grèce). Dumerilia 2 (1995): 23–54.
3. Bringsøe H. & Buskirk J.R. 1998. Distribution of Testudo kleinmanni Lortet, 1883 and Testudo graeca Linnaeus, 1758 in the Negev Desert, southern Israel. Faunist. Abh. Mus. Tierkd. Dresden 21(Suppl.): 23–30.
4. Carretero M.A., Znari M., Harris D.J. & Macé J.C. 2005. Morphological divergence among populations of Testudo graeca from west-central Morocco. Anim. Biol. 55: 259–279. CrossRef
5. Chkhikvadze V.M. 1988. O sistematicheskom polozhenii sovremennykh sukhoputnykh cherepakh Sredneĭ Azii i Kazakhstana. Izv. Akad. Nauk GSSR, Ser. Biol. 14: 110–113.
6. Chkhikvadze V.M., Amiranashvili N.G. & Ataev Ch. 1990. Novyĭ podvid sukhoputnoĭ cherepakhi iz yugo-zapadnogo Turkmenistana. Izv. Akad. Nauk Turkmensk. SSR, Ser. Biol. 1: 72–75.
7. Chkhikvadze V.M. & Bakradze M.A. 1991. O sistematicheskom polozhenii sovremennoĭ sukhoputnoĭ cherepakhi iz doliny reki Araks. Trudy Tbilissk. Gosud. Univ. 305: 59–63.
8. Chkhikvadze V.M. & Bakradze M.A. 2002. Novyĭ podvid sukhoputnoĭ cherepak hi iz Dagestana. Trudy Inst. Zool. Akad. Nauk Gruzii 21: 276–279.
9. Chkhikvadze V.M. & Tuniyev B.S. 1986. O sistematicheskom polozhenii sovremennoĭ sukhoputnoĭ cherepakhi zapadnogo Zakavakazya. Soobshch. Akad. Nauk Gruzinsk. SSR 124: 617–620.
10. Clement M., Posada D. & Crandall K.A. 2000. tcs: a computer program to estimate gene genealogies. Mol. Ecol. 9: 1657–1660. CrossRef
11. Crandall K., Templeton A.R. & Sing C.F. 1994. Intraspecific phylogenetics: problems and solutions, pp. 273–297. In: Scotland R.W., Siebert D.J. & Williams D.M. (eds), Models in Phylogeny Reconstruction, Clarendon Press, Oxford.
12. Fritz U., Široký P., Kami H. & Wink M. 2005. Environmentally caused dwarfism or a valid species — Is Testudo weissingeri Bour, 1996 a distinct evolutionary lineage? New evidence from mitochondrial and nuclear genomic markers. Mol. Phylogenet. Evol. 37: 389–401. CrossRef
13. Fritz U., Auer M., Bertolero A., Cheylan M., Fattizzo T., Hundsdörfer A.K., Martín Sampayo M., Pretus J.L., Široký P. & Wink M. 2006. A rangewide phylogeography of Hermann’s tortoise, Testudo hermanni (Reptilia: Testudines: Testudinidae): implications for taxonomy. Zoologica Scripta 35: 531–543.CrossRef
14. Fritz U., Hundsdörfer A.K., Široký P., Auer M., Kami H., Lehmann J., Mazanaeva L.F., Türkozan O. & Wink M. 2007. Phenotypic plasticity leads to incongruence between morphology-based taxonomy and genetic differentiation in western Palaearctic tortoises (Testudo graeca complex; Testudines, Testudinidae). Amphibia-Reptilia 28: 97–121. CrossRef
15. Fritz U. & Buskirk J.R. 1997. The occurrence of Testudo kleinmanni in Tripolitania. Čas. Národ. Muz., Řada Přírod. 167: 143–144.
16. Fu Y.-X. & Li W.-H. 1993. Statistical tests of neutrality of mutations. Genetics 133: 693–709.
17. Hall T.A. 1999. bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41: 95–98.
18. Harris D.J., Znari M., Macé J.-C. & Carretero M.A. 2003. Genetic variation in Testudo graeca from Morocco estimated using 12S rRNA sequencing. Rev. Esp. Herpetol. 17: 5–9.
19. Highfield A.C. 1990. Tortoises of north Africa; taxonomy, nomenclature, phylogeny and evolution with notes on field studies in Tunisia. J. Chelon. Herpetol. 1(2): 1–56.
20. Highfield A.C. & Martin J. 1989a. A revision of the Testudines of North Africa, Asia and Europe. Genus: Testudo. J. Chelon. Herpetol. 1(1): 1–12.
21. Highfield A.C. & Martin J. 1989b. Testudo whitei Bennett 1836. New light on an old carapace — Gilbert White’s Selborne tortoise re-discovered. J. Chelon. Herpetol. 1(1): 13–22.
22. Highfield A.C. & Martin J. 1989c. Description of a miniature tortoise Testudo flavominimaralis n. species from North Africa. The Tortoise Trust/Tortoise Survival Project, London, 4 pp.
23. Kumar S., Tamura K. & Nei N. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings Bioinform. 5: 150–163. CrossRef
24. Le M., Raxworthy C.J., McCord W.P. & Mertz L. 2006. A molecular phylogeny of tortoises (Testudines: Testudinidae) based on mitochondrial and nuclear genes. Mol. Phylogenet. Evol. 40: 517–531. CrossRef
25. Mayer R. 1992. Europäische Landschildkröten. Leben — Haltung — Zucht. Agrar Verlag Allgäu, Kempten, 127 pp.
26. Parham J.F., Macey J.R., Papenfuss T.J., Feldman C.R., Türkozan O., Polymeni R. & Boore J. 2006. The phylogeny of Mediterranean tortoises and their close relatives based on complete mitochondrial genome sequences from museum specimens. Mol. Phylogenet. Evol. 38: 50–64. CrossRef
27. Perälä J. 1996. Etelä-Turkin maakilpikonnista. Morfologisia ja ekologisia eroja (Testudo ibera Pallas 1814 & Testudo ibera anamurensis Weissinger 1987) sekä uuden maakilpikonnalajin kuvaus + 15 kuvaa, pp. 14–26. In: Perälä J., Vikberg J. & Kanza M. (eds), Virallinen kongressijulkaisu. Herpetokongressi I, Suomen herpetologinen yhdistys ry, Helsinki.
28. Perälä J. 2001. A new species of Testudo (Testudines: Testudinidae) from the Middle East, with implications for conservation. J. Herpetol. 35: 567–582.CrossRef
29. Perälä J. 2002a. The genus Testudo (Testudines: Testudinidae): phylogenetic inferences. Chelonii 3: